(for acetoacetic acid in urine) to about 10 mL of urine add 5 drops of 30 per cent acetic acid, 5 drops strong iodine solution, and 2 to 3 mL chloroform, and shake. The chloroform does not change color if diacetic acid is present, but becomes reddish violet in its absence. Uric acid also decolorizes iodine, and if much is present, double the amount of strong iodine solution should be used.
