a modification of Mann methyl blue-eosin stain that changes the dye proportions, buffers the dye mixture, and stains at 60°C; basophils are stained blue to black, acidophils are dark red, chromophobe granules are gray to pink, and erythrocytes are orange; with modification, the method is also useful for enterochromaffin cells, goblet cells, Paneth cells, and pancreatic islet cells.