Tyson test

(for bile acids in urine) 180 to 240 mL of urine are evaporated to dryness on the water bath. The residue is extracted with absolute alcohol, and to the extract 12 to 14 volumes of ether are added. The bile acids are precipitated, then are filtered off, dissolved in water, and the aqueous solution decolorized with animal charcoal.

Chemwatch
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