a technique used to demonstrate cytoplasmic granules, after Bouin or formalin-Zenker fixatives, using oxidized hematoxylin plus phloxine; in the pancreas, beta cells are blue, alpha and delta cells are red, and zymogen granules are red to unstained; in the pituitary, alpha cells are pink, beta cells and chromophobes are gray-blue, and nuclei are purple to blue.
